Standardisation of the test procedure for individual serum samples:

1. (a)

   a 1/150 pre-dilution18 of the OIEISS or a 1/2 pre-dilution of the OIEELISA_WPSS or a 1/16 pre-dilution of the OIEELISA_SPSS made up in a negative serum (or in a negative pool of sera) must give a positive reaction;

2. (b)

   a 1/600 pre-dilution of the OIEISS or a 1/8 pre-dilution of the OIEELISA_WPSS or a 1/64 pre-dilution of the OIEELISA_SPSS made up in a negative serum (or in a negative pool of sera) must give a negative reaction;

3. (c)

   the OIEELISA_NSS must always give a negative reaction.

Standardisation of the test procedure for pooled serum samples:

1. (a)

   a 1/150 pre-dilution of the OIEISS or a 1/2 pre-dilution of the OIEELISA_WPSS or a 1/16 pre-dilution of the OIEELISA_SPSS made up in a negative serum (or in a negative pool of sera) and again diluted in negative sera by the number of samples making up the pool must give a positive reaction;

2. (b)

   the OIEELISA_NSS must always give a negative reaction;

3. (c)

   the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of serum have been pooled.

Standardisation of the test procedure for pooled milk or whey samples:

1. (a)

   a 1/1 000 pre-dilution of the OIEISS or a 1/16 pre-dilution of the OIEELISA_WPSS or a 1/125 pre-dilution of the OIEELISA_SPSS made up in a negative serum (or in a negative pool of sera) and again diluted 1/10 in negative milk must give a positive reaction;

2. (b)

   the OIEELISA_NSS diluted 1/10 in negative milk must always give a negative reaction;

3. (c)

   the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of milk or whey have been pooled.