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Commission Regulation (EC) No 1353/2000Show full title
Commission Regulation (EC) No 1353/2000 of 26 June 2000 concerning the permanent authorisation of an additive and the provisional authorisation of new additives, new additive uses and new preparations in feedingstuffs (Text with EEA relevance)
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ANNEX IIIU.K.
| a 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5.0 ande 40 °C. | ||||||||
| b 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5.0 and 40 °C. | ||||||||
| c 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5.0 ande 40 °C. | ||||||||
| d 1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C. | ||||||||
| e 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C. | ||||||||
| f 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C. | ||||||||
| g 1 U is teh amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C. | ||||||||
| h 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 6,5 and 37 °C. | ||||||||
| i 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from casein substrate per minute at pH 7,5 and 40 °C. | ||||||||
| j 1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40 °C. | ||||||||
| l 1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5.5 and 37 °C. | ||||||||
| k 1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C. | ||||||||
| m 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 7.5 and 30 °C. | ||||||||
| n 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7.5 and 30 °C. | ||||||||
| o 1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7.4 and 37 °C. | ||||||||
| No.(or EC No) | Additive | Chemical formula, description | Species or category of animal | Maximum age | Minimum content | Maximum content | Other provisions | Period of authorisation |
|---|---|---|---|---|---|---|---|---|
| Units of activity/kg of complete feedingstuff | ||||||||
| 12 | Endo-1,4-beta-glucanase EC 3.2.1.4 | Preparation of endo-1,4-beta-glucanase, endo-1,3 (4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity | Turkeys for fattening | — | Endo-1,4-beta-glucanase 800 U | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed rich in non-strach polysaccarides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20 % wheat and 20 % barley. | 30.9.2001 |
| Endo-1,3(4)-beta-glucanase EC 3.2.1.6 | Endo-1,3(4)-beta-glucanase: 1 800 U | — | ||||||
| Endo-1,4-beta-xilanase EC 3.2.1.8 | Endo-1,4-beta-xylanase: 2 600 U | — | ||||||
| 17 | Endo-1,4-beta-xylanase EC 3.2.1.8 | Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
| Turkeys for fattening | — | 750 EPU | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 35 % wheat. | 30.9.2001 |
| 42 | Endo-1,4(4)-betaxylanase EC 3.2.1.8 | Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
Characteristic of the authorised preparation:
| Pigs for fattening | — | 4 000 U | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 60 % wheat. | 30.9.2001 |
| 49 | Endo-1,3(4)-beta-glucanase EC 3.2.1.6 | Preparation of endo-1,3(4)-beta-glucanase producted by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: | Chickens for fattening | — | Endo-1,3(4)-beta-glucanase: 150 U | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuffs:
3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat. | 30.9.2001 |
| Endo-1,4-beta-xilanasa EC 3.2.1.8 | Endo-1,4-beta-xylanase: 1 500 U | — | ||||||
| Alfa-amylase EC 3.2.1.1 | Alfa-amylase: 500 U | — | ||||||
| Bacillolysin EC 3.4.24.28 | Bacillolysin 800 U | — | ||||||
| Polygalacturonase C 3.2.1.15 | Polygalacturonase 50 U | — | ||||||
| Layinghens | — | endo-1,3(4)-beta-glucanase: 150 U | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuffs:
3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat. | 30.9.2001 | |||
| endo-1,4-beta-xylanase: 1 500 U | — | |||||||
| alpha-amylase: 1 000 U | — | |||||||
| bacillolysin: 800 U | — | |||||||
| polygalacturonase: 50 U | — | |||||||
| 50 | 6-phytase EC 3.1.3.26 | Preparation of 6-phytase produced by Aspergillus oryzae (DSM 11857) having a minimum activity of:
| Chickens for fattening | — | 250 FYT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed containing more than 0,25 % phytin bound phosphorus. | 30.9.2001 |
| Laying hens | — | 250 FYT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed containing more than 0,25 % phytin bound phosphorus. | 30.9.2001 | |||
| Turkeys for fattening | — | 250 FYT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed containing more than 0,25 % phytin bound phosphorus. | 30.9.2001 | |||
| Piglets | 2 months | 500 FYT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed containing more than 0,25 % phytin bound phosphorus. | 30.9.2001 | |||
| Pigs for fattening | — | 500 FYT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed containing more than 0,25 % phytin bound phosphorus. | 30.9.2001 | |||
| 51 | Endo-1,4-betaxylanase EC 3.2.1.8 | Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG-S 15136) having a minimum activity of:
| Chickens for fattening | — | 10 IU | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat. | 30.9.2001 |
| 52 | Endo-1,3(4)-beta-glucanase: EC 3.2.1.6 | Preparation of endo-1,3(4)-beta-glucanasa produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), having a minimum activity of: | Chickens for fattening | — | Endo-1,3(4)-beta-glucanase: 1 000 U | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.2.Recommended dose per kg of complete feedingstuff:
3.For use in compound feed rich in non starch polysaccharides (mainly arabinoxylans and betaglucans) e.g. containing more than 20 % wheat and 15 % sorghum and 5 % maize. | 30.9.2001 |
| Endo-1,4-beta-glucanase: EC 3.2.1.4 | Endo-1,4-beta-glucanase: 12 000 U | — | ||||||
| Alpha-amylase EC 3.2.2.1 | Alpha-amylase: 40 U | — | ||||||
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