SCHEDULE 3Sampling and Testing Methods

Part IIIMethods for the Isolation of Salmonella

A. Bacteriological Method

Day 46

The plates shall be examined and a minimum of 3 colonies from each plate showing suspicion of Salmonella growth shall be subcultured—

a

onto a nutrient agar plate;

b

onto a MacConkey agar plate27; and

c

into biochemical media suitable for the identification of Salmonella.

These media shall be incubated at 37°C ± 1°C overnight.