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21. Tests shall be carried out in duplicate using two 25 gram ± 1 gram portions of each sample submitted for testing. Each 25 gram ± 1 gram sample shall be placed aseptically in a container containing 225 ml ± 1 ml Buffered Peptone Water (BPW) and incubated at 37°C ± 1°C for 18 hours ± 2 hours.
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