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Animal By-Products Regulations (Northern Ireland) 2003

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27.  The RVS broth shall be plated out onto two 90 mm plates of Brilliant Green Agar (BGA)(1) or onto one 90 mm plate of BGA and one 90 mm plate of Xylose Lysine Deoxycholate Agar (XLD)(2) using a 2.5 mm diameter loop. The plates shall be inoculated with a droplet taken from the edge of the surface of the fluid by drawing the loop over the whole of one plate in a zig zag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks shall be 0.5 cm-1.0 cm. The plates shall be incubated at 37°C ± 1°C for 18 to 24 hours.

(1)

Brilliant Green Agar – See Edel, W and Kampelmacher, E.H. (1969) Bulletin of World Health Organisation, 41:297-306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).

(2)

Xylose Lysine Deoxycholate Agar – See Taylor, W.I. (1965) American Journal of Clinical Pathology, 44:471-475, Lippincott and Raven, 227 E. Washington Street Philadelphia PA19106, USA Back [2]

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