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Animal By-Products Regulations (Northern Ireland) 2003

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Samples with colonies of Enterobacteriaceae

51.  After incubation each set of duplicate plates shall be examined for colonies characteristic of Enterobacteriaceae (purple colonies 1-2 mm in diameter). All characteristic colonies on each plate shall be counted and the arithmetic mean of the duplicate plates taken.

The sample provisionally fails if either –

(a)any arithmetic mean is above 30(1); or

(b)three or more arithmetic means are above 10;

in which case the following procedure shall be followed to establish whether or not the colonies are Enterobacteriaceae.

(1)

An arithmetic mean of 30 is equivalent to 3 × 102 colony forming units per gramme of original sample

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