9. After incubation each plate shall be examined for colonies characteristic of Clostridium perfringens. At least 3 colonies characteristic of Clostridium perfringens shall be –
(a)stab inoculated into motility nitrate medium(1); and
(b)inoculated into either lactose gelatine medium(2)
and incubated anaerobically at 37°C ± 1°C for 20 hours ± 2 hours.
Motility nitrate medium – See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892. National Research Council of Canada, Ottawa ON K1A OR6, Canada
Lactose gelatine medium – See Hauschild AHW, Gilbert R J, Harmon S M, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892